genomic相关论文
Drought is one of the most important environmental constraints limiting plant growth,development and crop yield.Many dro......
Ten hepatitis B virus (HBV) genotypes (A-J) and 34 HBV subgenotypes have been identified so far. HBV genotypes and subge......
比较基因组杂交(comparativegenomic hybridization,CGH)是在染色体荧光原位杂交(fluorescence in situhybridization,FISH)基础上......
基因组印记 (genomic imprinting)是不符合孟德尔遗传定律的特殊遗传现象。巨大舌 -脐膨出综合征 (Beckwith- Wiedemann syndrome,......
miRNAs are a class of small, ~22nt, non-coding RNAs that negatively regulate gene expression at the post- transcriptiona......
AIM: To establish an accurate and rapid stem-loop reverse transcriptional real-time PCR (RT-PCR) method to quantify huma......
Invasive alien species (IAS) are species whose introduction to areas outside of their native range cause harm to economi......
The principles and characteristics of several novel molecular markers, SCAR (Sequence Characterized Amplified Region), I......
A major objective of quantitative trait locus (QTL) studies is to find genes/markers that can be used in breeding progra......
A major objective of quantitative trait locus (QTL) studies is to find genes/markers that can be used in breeding progra......
全基因组选择技术通过全基因组中大量的单核苷酸多态性标记(SNP)和参照群体的表型数据建立BLUP模型估计出每一标记的育种值,称为估......
Genetic diversity and a population structure analysis of accessions in the Chinese cowpea [Vigna ung
Cowpea(Vigna imguicuiata) is an important legume crop with diverse uses. The species is presently a minor crop, and eval......
A male-specific SCAR DNA marker was developed using a RAPD DNA marker specific for male plants of Salacca zalacca var. z......
Objective To evaluate whether mono(2‐ethylhexyl) phthalate(MEHP) affects genomic DNA methylation and the methylation st......
基因组组装是宏基因组分析的主要挑战之一。通常假设所有测序序列均来源于同一个基因组,微生物中非常活跃的可移动元件给这个前提假......
Abstract [Objective] This study aimed to detect the purity of Shanxi lean meat pigs. [Method] Eight primers were selecte......
用来自玉米(Zea mays L.)与二倍体多年生类玉米(Z. diploperennis Iltis, Doebley and Guzmán)杂交的孤雌生殖后代同一抗病个体的......
DNA聚合酶在DNA合成过程中需要的引物包括RNA引物、DNA自我引物和蛋白质引物3种类型。新DNA链(如冈崎片段)的复制多是在DNA模板上合......
To analyze the biological effects of space environment, the diversity of genomic DNA between the space flight soybean 19......
探讨了改良CTAB法、SDS法、高盐低pH法和试剂盒法对浙贝母(Fritillaria thunbergii Miq.)基因组DNA的提取效果。综合比较了各方法提......
4种经优化的DNA提取方法即CTAB区室法、SDS区室法、CTAB常规法、SDS常规法提取猕猴桃嫩叶基因组DNA,效果均好.经综合对比分析,本实......
随着全基因组时代的到来,高通量测序在动物新基因和分子标记挖掘方面的优势 日益突出,与此同时,全基因组关联分析( Genome-wide ass......
本研究采用乙酰基亚硝基脲(ENU)浸泡的方法诱变银鲫(Carassius autatus gibeblio Bloch)持续2周,后经清水反复漂洗,再进行人工催产得到......
To detect genetic alterations in nasopharyngeal carcinoma (NPC) in Cantonese, the population with the highest incidence ......
以已公布的黑麦胞质核糖体蛋白基因ScRPS7的cDNA序列为信息探针,在中国华大水稻基因组数据库中搜索与之高度同源的基因组重叠群.采......
猪繁殖与呼吸综合征(PRRS)是由猪繁殖与呼吸综合征病毒(PRRSV)引起的一种传染病,该病主要引起妊娠母猪流产、死胎、木乃伊胎、弱仔等繁......
介于蓖麻叶片富合多糖、多酚等大分子物质,DNA的提取比较困难,文中总结性介绍了近年来提取蓖麻基因组DNA的几种主要方法:CTAB法、SDS......
以天竺葵为试材,比较分析了CTAB法、简单法、高盐低pH值法、CTAB-SDS法、SDS法对天竺葵叶基因组DNA提取的效果。结果表明:5种方法提......
利用热启动PCR法分别从矮紫杉、欧洲红豆杉以及中国红豆杉基因组DNA中首次克隆到长度为1456bp的BAPT基因全长序列。序列分析结果表......
研究表明八氢番茄红素合成酶(PSY)是巴氏杜氏藻(Dunaliella bardawil)类胡萝卜素代谢途径中的第一个关键调节酶。本实验采用基于LA-PCR......
慢性髓系白血病(chronic myelogenous leukemia,CML)是一种骨髓增殖性疾病,其主要特点是髓系细胞的过度增殖。患者往往早期(即慢性......
利用自行设计的方法分离提取总RNA,通过一对特异引物,扩增出苹果锈果类病毒的全长片段,该片段通过回收、克隆和测序,结果发现该片段全......
乳酸菌具有黏附宿主细胞或黏膜、抑制病原菌生长、维持机体微生态平衡、增强宿主免疫力等促进宿主健康的作用,同时在食品工业、抗......
选择2种简化的棉花基因组DNA提取方法并进行适当精简改良后与传统CTAB提取法进行实验对比的结果显示,改良SDS-CTAB法所提DNA得率较......
利用细胞核移植技术将NIH3T3细胞核和孤雌桑椹胚单个卵裂球,分别移植到去核MⅡ期受体卵母细胞中,通过免疫荧光染色后比较体外受精胚......
简要叙述了表达序列标签EST技术的原理,对小麦EST图谱的构建研究进展进行了综述,分别阐述了小麦七个同祖群染色体的EST图谱的研究进......
Ribosonal protein L21 which is a component of the 60s large ribosomal subunit plays an important role in ribosome. To ex......
In germinating wheat embryos, gl-OXO accumulation is localized in cell wall. It has been confirmed that this enzyme loca......